Journal:

Article Title: In Vitro Modeling of Repetitive Motion Injury and Myofascial Release

doi: 10.1016/j.jbmt.2010.01.002

Figure Lengend Snippet: Representative photomicrographs of human fibroblast construct morphology, growth patterns and actin architecture of the four treatment groups: Control, repetitive motion strain (RMS), myofascial release (MFR), and RMS+MFR.

Article Snippet: Human Fibroblast Cultures Normal human dermal fibroblasts (NHDF) from Cambrex Laboratories (East Rutherford, New Jersey) were used for all experiments.

Techniques: Construct, Control

Journal:

Article Title: In Vitro Modeling of Repetitive Motion Injury and Myofascial Release

doi: 10.1016/j.jbmt.2010.01.002

Figure Lengend Snippet: NHDF secretion of select cytokines and growth factors from the four treatment groups described. Group means and SEMs are calculated with the exclusion of significant outliers determined by Grubbs’ Test. All data are expressed in pg/ml (N=2-3)

Article Snippet: Human Fibroblast Cultures Normal human dermal fibroblasts (NHDF) from Cambrex Laboratories (East Rutherford, New Jersey) were used for all experiments.

Techniques: Control

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Enabling 3D bioprinting of cell-laden pure collagen scaffolds via tannic acid supporting bath

doi: 10.3389/fbioe.2024.1434435

Figure Lengend Snippet: (A) Live/dead representative images of nHDFs post-bioprinting. On day 1 a considerable number of dead cell nuclei is visible for both conditions. However, cell viability significantly increases on days 3 and 7. Overall, a homogeneous cell distribution is observed, with no signs of cell agglomeration (B) Ratio between alive and dead cells post-bioprinting. Cell counting confirmed the mortality observed in the live/dead 3D images . It was noticed a general tendency of increasing cell viability during in vitro culture for both conditions. * p < 0.05, *** p < 0.001, n = 3.

Article Snippet: Neonatal human dermal fibroblasts (nHDFs), high glucose Dulbecco’s Modified Eagle Medium (DMEM), Penicillin/Streptomycin, Hepes and fetal bovine serum (FBS) were purchased from ThermoFisher Scientific (United States).

Techniques: Cell Counting, In Vitro

Journal: International Journal of Molecular Sciences

Article Title: Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding Peptides

doi: 10.3390/ijms25031462

Figure Lengend Snippet: Solution peptide effect on NHDF viability assessed through metabolic activity. All data are normalized to the metabolic activity of untreated control cells indicated by the red dashed line; 70% activity is indicated by the grey dashed line. Data are presented as the mean and standard deviation from three independent experiments ( n = 3 per experiment). Statistical differences determined by an ANOVA followed by the Tukey’s HSD test (* p < 0.05, ** p < 0.01, *** p < 0.005).

Article Snippet: Komagataeibacter hansenii NQ5 ( K. hansenii ATCC 53582), normal human dermal fibroblasts (NHDFs; American Type Culture Collection: CRL-2565), Staphylococcus aureus (ATCC 43866, strain designation: LRA 44.01.83), Escherichia coli (ATCC 33694, strain designation: HB101) and Pseudomonas aeruginosa (ATCC 29260, strain designation: PA-103) were purchased from the American Type Culture Collection (ATCC), Manassas, VA, USA, while human endothelial keratinocyte cells (HaCaT, catalog number: T0020001) were purchased from AddexBio, San Diego, CA, USA.

Techniques: Activity Assay, Control, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Functionalization of Bacterial Cellulose with the Antimicrobial Peptide KR-12 via Chimerical Cellulose-Binding Peptides

doi: 10.3390/ijms25031462

Figure Lengend Snippet: Functionalized BC effect on NHDF and HaCaT viability. Normalized metabolic activity of ( A , B ) NHDF and ( C , D ) HaCaT after exposure to unfunctionalized BC (BC control) and BC functionalized with Long-CBP-KR12 and Short-CBP-KR12 normalized to untreated cells. Both the ( A , C ) opaque and ( B , D ) transparent BC were evaluated. All data are normalized to the metabolic activity of untreated control wells indicated by the red dashed line, and 70% activity is indicated by the grey dashed line. Data are presented as the mean and standard deviation from three independent experiments ( n = 3 per experiment). No statistical differences determined by ANOVA.

Article Snippet: Komagataeibacter hansenii NQ5 ( K. hansenii ATCC 53582), normal human dermal fibroblasts (NHDFs; American Type Culture Collection: CRL-2565), Staphylococcus aureus (ATCC 43866, strain designation: LRA 44.01.83), Escherichia coli (ATCC 33694, strain designation: HB101) and Pseudomonas aeruginosa (ATCC 29260, strain designation: PA-103) were purchased from the American Type Culture Collection (ATCC), Manassas, VA, USA, while human endothelial keratinocyte cells (HaCaT, catalog number: T0020001) were purchased from AddexBio, San Diego, CA, USA.

Techniques: Activity Assay, Control, Standard Deviation